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Catalog Number | orb614117 |
---|---|
Category | Antibodies |
Description | GLB1/Beta-galactosidase Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human GLB1/Beta-galactosidase recombinant protein (Position: Q46-K655). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | "Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human" |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 65-85 kDa |
UniProt ID | P16278 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Beta-galactosidase; Acid beta-galactosidase; Lacta Read more... |
Note | For research use only |
Application notes | "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of MCF-7 cells using anti-GLB1/Beta-galactosidase antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
IF analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody.
IHC analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody.
IHC analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody.
WB analysis using anti-GLB1/Beta-galactosidase antibody.Lane 1:HeLa cell, Lane 2:HepG2 cell, Lane 3:SH-SY5Y cell, Lane 4:MCF-7 cell.
ELISA, FC, ICC, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IHC-P, WB | |
Canine, Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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