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Giantin Rabbit Polyclonal Antibody

Catalog Number: orb157139

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb157139
CategoryAntibodies
DescriptionGiantin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityCanine, Equine, Hamster, Monkey, Mouse, Rabbit
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Giantin (751-850/3259aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW376 kDa
TargetGOLGB1
UniProt IDQ14789
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names372 kDa Golgi complex associated protein; 372 kDa
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NoteFor research use only
Expiration Date12 months from date of receipt.
Giantin Rabbit Polyclonal Antibody

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Giantin Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Giantin antibody (orb157139), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Giantin Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Giantin antibody (orb157139), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Giantin Rabbit Polyclonal Antibody

Blank control: Lovo Cells (blue). Primary Antibody: Rabbit Anti-Giantin/AF488 Conjugated antibody, Dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

Giantin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervix cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (orb157139) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Giantin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervix cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (orb157139) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Giantin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (orb157139) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Giantin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (orb157139) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Giantin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Giantin) Polyclonal Antibody, Unconjugated (orb157139) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

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    100 μl