Ghrelin 28 Rabbit Polyclonal Antibody

• Catalog Number: orb11180
• Category: Antibodies
• Description: Ghrelin 28 Rabbit Polyclonal Antibody
• Target: GHRL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Human, Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide of mouse Ghrelin 28 (24-51/117aa)
• RRID: AB_10753020
• MW: 13 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Appetite regulating hormone; Ghrelin 27; Ghrelin 2
• Research Area: Cardiovascular Research, Diabetes Research, Hormon
• Note: For research use only

References

Li Xu 1, Jie Su 2, Caili Du 3, Hui Liu 4, Wei Lv Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3 Cell Mol Biol (Noisy-le-grand), (2024)

PubMed: 38158667

Applications

WB

Reactivity

Rat

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Ghrelin 28) Polyclonal Antibody, Unconjugated (orb11180) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.