GFP11-tag Antibody

SKU: orb621407

Description

GFP11-tag Antibody. Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide RDHMVLHEYVNAAGITC linked to KLH.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1*PBS (pH7.4), 0.2% rAlbumin, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

WB analysis of GFP11-tag on fusion protein using GFP11-tag Antibody at 1/1,000 dilution.

Silvia Marchese Establishing an In Vitro Model for CRISPR-Cas9 Screening of Interferon Effectors in HBV Infection UNIVERSITÀ DEGLI STUDI DI MILANO, Department of Pharmacological and Biomolecular Sciences, (2025)

PubMed:

Applications

WB

Reactivity

Human