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GFP Tag Nanobody Immunomagnetic Beads
Catalog Number: orb2946031
| Catalog Number | orb2946031 |
|---|---|
| Category | Small Molecules |
| Description | Biorbyt's GFP Tag Nanobody Immunomagnetic Beads specifically bind to proteins with the GFP tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.Nanobody is a class of variable region fragments (VHH) of natural heavy chain antibodies derived from camelids (e.g. llamas, alpacas), which are the smallest known natural functional antibody structural units, usually only about 12-15 kDa, one-tenth of the size of traditional IgG antibodies. Nanoantibodies are smaller than traditional IgG antibodies, have higher affinity, no interference from light and heavy chains, can be closer to the target protein epitopes and reduce the spatial site resistance, and at the same time, are not easy to be detached, and the elution conditions are mild, so that they can maintain higher biological activity on the samples. |
| Storage | -20°C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
