GFP Control protein

• Catalog Number: orb345957
• Category: Proteins
• Description: GFP Control protein
• Tested applications: ELISA, IP, SDS-PAGE, WB
• Concentration: 1mg/ml
• Dilution range: ELISA: User Optimized, IP: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: GFP control protein was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.
• Conjugation: Unconjugated
• UniProt ID: P42212
• Storage: Store GFP at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: control protein, rGFP protein, GFP fusion protein,
• Note: For research use only
• Application notes: GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa. Green Fluorescent Protein has been tested in SDS-page, western blot, and immunoprecipitation.
• Expiration Date: 6 months from date of receipt.

Western blot analysis of 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates using GFP Control protein