GFP antibody

• Catalog Number: orb345329
• Category: Antibodies
• Description: GFP antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9F9.F9
• Tested applications: DOT, ELISA, FC, IF, IHC, IP, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:30,000, FC: User Optimized, IHC: 1:1,000 - 1:5,000, IF: User Optimized, WB: 1:3,000 - 1:30,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
• Conjugation: Unconjugated
• UniProt ID: P42212
• Storage: Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-GFP antibody, Green Fluorescent Protein
• Note: For research use only
• Application notes: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng using GFP antibody

Western blot analysis of HeLa cell-derived lysates using GFP antibody