GFP Antibody

SKU: orb11604

Description

Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Purified recombinant peptide produced in E. coli
• Target: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins
• Purity: This antibody is epitope-affinity purified from goat antiserum
• Purification: Epitope affinity purified

Storage & Handling

• Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Green fluorescent protein antibody

Primary fibroblasts transduced with ad GFP-Ra1a and immunostained with GFP antibody

AtT-20 transfected with GFP-Rab27a and immunostained with GFP antibody

Western blot analysis of HEK293 transfered cell lysates at 50 ug per lane using GFP antibody

Quick Database Links

Gene Symbol

In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins