GFP antibody

• Catalog Number: orb11604
• Category: Antibodies
• Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Isotype: IgG
• Immunogen: Purified recombinant peptide produced in E. coli
• Concentration: 2 mg/ml
• Dilution range: WB:1:500-1:5,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000
• Form/Appearance: PBS, 20% glycerol and 0.05% sodium azide
• Purity: This antibody is epitope-affinity purified from goat antiserum
• Conjugation: Unconjugated
• Target: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins
• Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: Green fluorescent protein antibody
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Primary fibroblasts transduced with ad GFP-Ra1a and immunostained with GFP antibody

AtT-20 transfected with GFP-Rab27a and immunostained with GFP antibody

Western blot analysis of HEK293 transfered cell lysates at 50 ug per lane using GFP antibody