GFP antibody (FITC)

• Catalog Number: orb345838
• Category: Antibodies
• Description: GFP antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, FC, IF, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:40,000, FC: 1:2000, IF: 1:500 - 1:2,500, WB: 1:10,000-1:25,000
• Form/Appearance: Lyophilized
• Purity: GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
• Conjugation: FITC
• UniProt ID: P42212
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-GFP antibody fluorescein conjugation,
• Note: For research use only
• Application notes: Anti-Green fluorescent protein Fluorescein conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Biorbyt FITC conjugated Rabbit anti GFP (green) stains mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells In Vivo.

Western Blot of GFP Antibody Fluorescein Conjugated. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.