GFP antibody (Biotin)

• Catalog Number: orb345877
• Category: Antibodies
• Description: GFP antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, IF: 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
• Conjugation: Biotin
• UniProt ID: P42212
• Storage: Store Anti-GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-GFP antibody biotin conjugation, bioti
• Note: For research use only
• Application notes: Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Biotin Mouse with 1:5000 using GFP antibody (Biotin)

Western blot analysis of Lane 1: 50ng of GFP. Lane 2: none using GFP antibody (Biotin)