GFM2 Rabbit Polyclonal Antibody

• Catalog Number: orb157115
• Category: Antibodies
• Description: GFM2 Rabbit Polyclonal Antibody
• Target: GFM2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human GFM2 (151-250/779aa)
• UniProt ID: Q969S9
• MW: 87 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EF-G2mt; EFG2; EFG2mt; elongation factor G 2, mito
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GFM2) Polyclonal Antibody, Unconjugated (orb157115) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Protein: A549 (human) lyates at 40 ug, Primary: Rabbit Anti-GFM2 (orb157115) at 1:300, Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG (H+L) at 1: 10000, Predicted band size: 87 kD, Observed band size: 63 kD.