GFAP Rabbit Polyclonal Antibody

SKU: orb1675372

Description

GFAP Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution range: WB=1:5000-20000, ICC/IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human GFAP protein (1-390/432aa)
• Target: GFAP
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.

25 ug total protein per lane of various lysates probed with GFAP polyclonal antibody, unconjugated (orb1675372) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Measured by its binding ability in a indirect ELISA. Immobilized Human GFAP protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human GFAP Antibody, the EC50 is 540.1 ng/ml.

Quick Database Links

Gene Symbol

GFAP

UniProt

P14136