GFAP Antibody / Glial Fibrillary Acidic Protein

• Catalog Number: orb2636196
• Category: Antibodies
• Description: This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFAP/9421
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: A recombinant fragment (within amino acids 150-250) of human GFAP protein was used as the immunogen for the GFAP antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GFAP antibody is available for research use only.
• UniProt ID: P14136
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the GFAP antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain tissue lysate with GFAP antibody (clone GFAP/9421). Predicted molecular weight ~50 kDa.

IHC staining of FFPE human prostate carcinoma with GFAP antibody (clone GFAP/9421). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GFAP antibody (clone GFAP/9421) as confirmation of integrity and purity.