GDN Rabbit Polyclonal Antibody

SKU: orb1120

Description

GDN Rabbit Polyclonal Antibody

Research Area

Cell Biology, Fbrinolysis / Thrombolysis, Neurogenesis, Neuroscience, Protease, Varscular

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GDN/SERPINE2 (301-398/398aa)
• Target: SERPINE2
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Glia derived nexin; Protease inhibitor 7; GDN; GDN_HUMAN; Glia-derived nexin; P17 antibody Peptidase inhibitor 7; Pi-7; Plasminogen activator inhibitor type 1, member 2; PN-1; PN1; PNI; Protease nexin 1; Protease nexin I; Serpin E2; Serpin peptidase inhibitor, clade E(nexin, plasminogen activator inhibitor type 1), member 2; SERPINE 2; Serpine2.

Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GDN) Polyclonal Antibody, Unconjugated (orb1120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GDN) Polyclonal Antibody, Unconjugated (orb1120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Bone (Mouse) Lysate at 40 ug, Skeletal muscle (Mouse) Lysate at 40 ug, Primary: Anti-GDN (orb1120) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Spinal cord (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-GDN (orb1120) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Quick Database Links

Gene Symbol

SERPINE2

UniProt

P07093