GDF9 Antibody / Growth Differentiation Factor 9

• Catalog Number: orb2636459
• Category: Antibodies
• Description: GDF9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9/6458 can be used in assays to detect oocyte expression and has been shown to neutralize GDF9 biological activity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GDF9/6458
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant fragment (within amino acids 250-450) of human Growth Differentiation Factor 9 protein was used as the immunogen for the Growth Differentiation Factor 9 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Growth Differentiation Factor 9 antibody is available for research use only.
• UniProt ID: O60383
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the Growth Differentiation Factor 9 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human ovary tissue with Growth Differentiation Factor 9 antibody (clone GDF9/6458). Inset: PBS used in place of primary Ab (secondary Ab negative control).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Growth Differentiation Factor 9 antibody (clone GDF9/6458). These results demonstrate the foremost specificity of the GDF9/6458 mAb. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (clone MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free Growth Differentiation Factor 9 antibody (clone GDF9/6458) as confirmation of integrity and purity.