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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to inhibit the GCSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 for this effect is typically 1-5μg /ml in the presence of 0. 125 ng /ml of recombinant human GCSF. |
| Target | CSF3R |
| Tag | C-Fc Tag |
| MW | 92.8 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Glu 25 - Pro 621 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CSF3R, CD114, GCSFR
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Human CD114 Protein, His Tag [orb689426]
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The protein has a predicted molecular mass of 67.5 kDa after removal of the signal peptide.The apparent molecular mass of CD114-His is approximately 100-110 kDa due to glycosylation.
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1 mg, 50 μg, 100 μgRecombinant Human CD114/CSF3R Protein, N-His [orb2967446]
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Human G-CSF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000751.1 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
GCSFR Recombinant Protein (orb1228043)
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