GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

• Catalog Number: orb699488
• Category: Antibodies
• Description: GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.
• UniProt ID: P48507
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the GCLM antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CPTC-GCLM-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This GCLM antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.

SDS-PAGE analysis of purified, BSA-free GCLM antibody as confirmation of integrity and purity.