GCLC Antibody

• Catalog Number: orb215955
• Category: Antibodies
• Description: GCLC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 72766 MW
• UniProt ID: P48506
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutamate--cysteine ligase catalytic subunit;6.3.2
• Note: For research use only
• Application notes: WB: The detection limit for GCLC is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of GCLC using anti-GCLC antibody.Lane 1:recombinant human GCLC protein 0.5 ng.

WB analysis of GCLC using anti-GCLC antibody.Lane 1:rat brain tissue; 2:rat cardiac muscle tissue; 3:human HeLa cell; 4:rat PC-12 cell; 5:rat NRK cell; 6:human HEPA cell; 7:human A549 cell.

IHC analysis of GCLC using anti-GCLC antibody. GCLC was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of GCLC using anti-GCLC antibody. GCLC was detected in a paraffin-embedded section of human mammary cancer tissue.