GCK Antibody (N-term)

• Catalog Number: orb1928780
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB05442
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 52191 Da
• Target: This GCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCK.
• UniProt ID: P35557
• NCBI: NP_000153.1, NP_277042.1, NP_277043.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Glucokinase, Hexokinase type IV, HK IV, Hexokinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-GCK Pab is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).

GCK Antibody (M1) western blot analysis in HepG2 cell line and rat liver lysates (35 ug/lane).This demonstrates the GCK antibody detected the GCK protein (arrow).

Confocal immunofluorescent analysis of GCK Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.