GCH1 antibody

• Catalog Number: orb19890
• Category: Antibodies
• Description: Goat polyclonal antibody to GCH1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 27.9; 25.8; 23.5
• Target: GCH1
• Entrez: 2643
• Protein Sequence: GKVHIGYLPNKQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GCH1 antibody, anti GTP cyclohydrolase 1 (dop
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 37kDa band observed in Human Lymph Node, Thymus and Tonsil lysates. Customer's experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19890 (1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using GCH1 antibody

Immunohistochemical staining of Human Cerebellum using GCH1 antibody