GBP1 Antibody / Guanylate binding protein 1

• Catalog Number: orb1824602
• Category: Antibodies
• Description: GBP1 (guanylate binding protein 1) is a 592 amino acid protein member of the GTPase protein family and is able to bind specifically to guanine nucleotides such as GMP, GDP and GTP. GMP is hydrolyzed to GTP in two consecutive cleavage steps, both of which are carried out by GBP1. Localized to the cytoplasm, GBP1 is expressed in endothelial cells of the vascular system and is induced by IFN-g during macrophage induction. GBP1 is thought to regulate the expression of MMP-1, which mediates the proliferation and invasiveness of endothelial cells. GBP1 plays a key role in regulating inflammatory cytokines and provides protection against vesicular stomatitis and encephalomyocarditis viruses. GBP1 expression is highly induced in the vessels of skin diseases such as psoriasis and Kaposi s sarcoma, making it a novel cellular activation marker that characterizes inflammatory cytokines of endothelial cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GBP1/7618
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant partial protein sequence (within amino acids 400-592) from the human protein was used as the immunogen for the GBP1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This GBP1 antibody is available for research use only.
• UniProt ID: P32455
• Storage: Aliquot the GBP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with GBP1 antibody (clone GBP1/7618). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GBP1 antibody (clone GBP1/7618) as confirmation of integrity and purity.