GBA Rabbit Polyclonal Antibody

SKU: orb157057

Description

GBA Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GBA (141-240/536aa)
• Target: GBA
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Glucosidase beta; Acid beta glucosidase; Acid beta-glucosidase; Alglucerase; beta glucocerebrosidase; BETA GLUCOSIDASE, ACID; beta-glucocerebrosidase; betaGC; D glucosyl N acylsphingosine glucohydrolase; D-glucosyl-N-acylsphingosine glucohydrolase; EC 3.2.1.45; Gba protein; GBA1; GC antibody GCase; GCB; GLCM_HUMAN; GLUC; Glucocerebrosidase(alt.); Glucocerebrosidase; GLUCOCEREBROSIDASE PSEUDOGENE; Glucosidase beta; Glucosidase, beta, acid; Glucosidase, beta; acid(includes glucosylceramidase); Glucosylceramidase; Imiglucerase; Lysosomal glucocerebrosidase.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GBA) Polyclonal Antibody, Unconjugated (orb157057) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with GBA Polyclonal Antibody, Unconjugated (orb157057) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: HepG2 (Human) Cell Lysate at 40 ug, Primary: Anti-GBA (orb157057) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Quick Database Links

Gene Symbol

GBA

UniProt

P04062