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GATA2 Rabbit Polyclonal Antibody

Catalog Number: orb101321

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb101321
CategoryAntibodies
DescriptionGATA2 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Gallus, Human, Porcine, Rabbit, Sheep
ReactivityMouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GATA-2 (351-450/480aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW53 kDa
TargetGATA2
UniProt IDP23771
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesGATA-2; GATA 2; GATA Binding Protein 2; GATA-bindi
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NoteFor research use only
Expiration Date12 months from date of receipt.
GATA2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GATA2) Polyclonal Antibody, Unconjugated (orb101321) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GATA2 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GATA2) Polyclonal Antibody, Unconjugated (orb101321) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

GATA2 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Liver tissue lysates, Primary: Anti-GATA2 (orb101321) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kDa, Observed band size: 55 kDa.

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