Gata1 Antibody

SKU: orb345825

Description

Gata1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:15,000 - 1:75,000, WB: 1:500 - 1:2,000
• Reactivity: Mouse
• Application Notes:
  Anti-Gata1 Antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse Gata1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.
• Target: Gata1
• Purity: Anti-Gata1 is directed against mouse Gata1. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.73 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Gata1 antibody, Gata-1, gata 1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GF-1, NF-E1 DNA-binding protein, Gf-1

Western Blot of Rabbit Anti-Gata1 antibody. Lane 1: NIH/3T3. Lane 2: mouse brain. Load: 25 µg per lane. Primary antibody: Gata1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 60 min at RT. Block: orb348637 Fluorescent Blocking Buffer used overnight at 4°C. Predicted/Observed size: 42.7 kDa for Gata1.

Quick Database Links

Gene Symbol

Gata1

UniProt

P17679

RefSeq (Protein):NP_032115.1