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Catalog Number | orb101742 |
---|---|
Category | Antibodies |
Description | GAS 6 Rabbit Polyclonal Antibody |
Target | GAS6 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Predicted Reactivity | Bovine, Canine, Equine, Gallus, Porcine, Rat |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide derived from human GAS 6 (551-650/721aa) |
UniProt ID | Q14393 |
MW | 76 kDa |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | AXL receptor tyrosine kinase ligand; AXL stimulato Read more... |
Note | For research use only |
Sample: Liver (Mouse) Lysate at 40 ug, Kidney (Mouse) Lysate at 40 ug, Primary: Anti-GAS 6 (orb101742) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 76 kD, Observed band size: 76 kD.
Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-GAS6 Polyclonal Antibody, Unconjugated (orb101742) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.