GAPDH Antibody

SKU: orb420356

Description

GAPDH antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 5.0 µg/ml, WB: 1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-GAPDH Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33S.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: GAPDH Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH).
• Purity: Anti-GAPDH Antibody is directed against human GAPDH protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.17
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-GAPDH antibody, rabbit anti-Glyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH Loading Control Antibody, Anti-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

Western Blot of Rabbit anti-GAPDH antibody. Lane 1: Opal Pre-stained ladder. Lane 2: NIH/3T3 Lysate (p/n orb348714). Lane 3: HEK293 lysate (p/n orb348669). Lane 4: MOLT-4 Lysate (p/n orb348696). Lane 5: A549 Lysate (p/n orb348675). Lane 6: HeLa Lysate (p/n orb348668). Lane 7: NIH 3T3 Nuclear Lysate (orb348724). Lane 8: HeLa Nuclear Lysate (p/n orb348671). Lane 9: Jurkat Lysate (p/n orb348674). Lane 10: Opal Pre-stained ladder. Load: 35 µg per lane. Primary antibody: GAPDH antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 30 min at RT. Blocking Buffer: orb348637 for 30 min at RT. Predicted/Observed size: 36 kDa for GAPDH.

Quick Database Links

UniProt

P04406

RefSeq:7669492