GAPDH antibody

SKU: orb555879

Description

Rabbit polyclonal antibody to GAPDH

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Dilution range: WB: 1:5000-1:100000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Reactivity: Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish
• Application Notes:
  Positive control: PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , E.coli M15

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
• Target: GAPDH
• Molecular Weight: 36
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS, 1% rAlbumin, 20% Glycerol (pH7)
• Concentration: 0.69 mg/ml
• Disclaimer: For research use only

Alternative Names

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Immunofluorescence analysis of HeLa cells using GAPDH antibody

Immunohistochemical staining of human salivary gland cancer using GAPDH antibody

Immunohistochemical staining of mouse intestine using GAPDH antibody

Immunohistochemical staining of rat testis using GAPDH antibody

Immunoprecipitation analysis of 293T whole cell using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of Whole cell extract using GAPDH antibody

Western blot analysis of Various whole cell using GAPDH antibody

Western blot analysis of PC-12 whole cell lysate(Lane 1), Rat2 whole cell lysate(Lane 2) using GAPDH antibody

Quick Database Links

Gene Symbol

GAPDH

UniProt

P04406

References

Hu Zheng 1, Liuyang Wu 1, Huisheng Yuan miR-30b-5p targeting GRIN2A inhibits hippocampal damage in epilepsy Open Med (Wars), 18, 20230675 (2023)

PubMed: 37016703

Applications

WB

Reactivity

Mouse