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Catalog Number | orb11616 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep |
Isotype | IgG |
Immunogen | Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli. |
Concentration | 2 mg/ml |
Dilution range | WB:1:500-1:10,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000 |
Form/Appearance | Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum. |
Conjugation | Unconjugated |
Target | GAPDH |
Storage | Store at -20°C for long-term storage. Store at 2-8°C for up to one month. |
Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
Alternative names | glyceraldehyde 3-phosphate dehydrogenase, glyceral Read more... |
Note | For research use only |
Application notes | The antibody solution should be gently mixed before use. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of 293 HEK cell lysate using GAPDH antibody
Immunofluorescence analysis of Hepa1-6 cells using GAPDH antibody at 1:50 dilution.
ICC, IHC-P, IP, WB | |
Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-P, WB | |
Canine, Gallus, Monkey, Rabbit, Sheep | |
Hamster, Human, Mouse, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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