GAPDH Antibody Loading Control

SKU: orb248123

Description

Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 3-5ug/ml
• Reactivity: Canine, Human, Mouse, Primate, Rat
• Application Notes:
  Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: GAP259
• Immunogen: A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.
• Purification: Protein G Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

Western blot testing of human samples with GAPDH antibody at 0.5 ug/ml.

Western blot testing of mouse samples with GAPDH antibody at 0.5 ug/ml.

Western blot testing of human/mouse samples with GAPDH antibody at 0.5 ug/ml.

IHC testing of FFPE human liver tissue with GAPDH antibody at 3 ug/ml.