gamma-H2AX Antibody

• Catalog Number: orb1530715
• Category: Antibodies
• Description: gamma-H2AX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 140
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: gamma-H2AX
• UniProt ID: P16104
• NCBI: NP_002096.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: H2AX histone; histone H2AX; H2AX; H2A/X; H2A.X; hi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse gamma H2AX by western blot.Samples: Whole cell lysate from NIH 3T3 cells. Antibody: Affinity purified rabbit anti-gamma H2AX antibody at 1:1000. Lower Panel: Rabbit anti-GAPDH recombinant monoclonal antibody.

Detection of human gamma H2AX by western blot.Samples: Whole cell lysate from Jurkat cells. Antibody: Affinity purified rabbit anti-gamma H2AX antibody at 1:1000. Lower Panel: Rabbit anti-H2AX recombinant monoclonal antibody.