Gamma Catenin Antibody / Plakoglobin

• Catalog Number: orb639493
• Category: Antibodies
• Description: It recognizes a protein of 80-87kDa, identified as gamma-Catenin or Plakoglobin. The catenins (α, β, γ and δ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. α T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. α T-catenin binds to α E-catenin as well as to β-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for α-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 11E4.
• Tested applications: FACS, IF, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full length human gamma Catenin fused to maltose binding protein was used as the immunogen for the Plakoglobin antibody. The epitope has been found to be in the N-terminal region.
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Flow cytometry: 1-2ug/million cells
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Plakoglobin antibody is available for research use only.
• UniProt ID: P14923
• Storage: Store the Plakoglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.