Gamma-Adaptin Rabbit Polyclonal Antibody

SKU: orb10106

Description

Gamma-Adaptin Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Gamma-Adaptin (721-822/822aa)
• Target: AP1G1
• Molecular Weight: 91 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Adapter-related protein complex 1 subunit gamma-1; Adaptor protein complex AP 1 gamma 1 subunit; Adaptor protein complex AP 1 subunit gamma 1; Adaptor protein complex AP-1 subunit gamma-1; Adaptor related protein complex 1 gamma 1 subunit; adaptor-related protein complex 1 gamma 1 subunit; ADTG; ADTG; AP 1 complex subunit gamma 1; AP-1 complex subunit gamma-1; AP1G1; AP1G1_HUMAN; CLAPG1; CLAPG1; Clathrin assembly protein complex 1 gamma 1 large chain; Clathrin assembly protein complex 1 gamma large chain; Clathrin assembly protein complex 1 gamma-1 large chain; Clathrin associated/assembly/adaptor protein large gamma 1; clathrin-associated/assembly/adaptor protein large gamma 1; gamma1 adaptin; gamma1-adaptin; Golgi adaptor HA1/AP1 adaptin gamma subunit; Golgi adaptor HA1/AP1 adaptin subunit gamma 1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; MGC18255.

Cell culture (MCF7), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

Cell culture (SW480), Paraformaldehyde-fixed, Incubated by 0.3% Triton-100 for 20 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (orb868805) for 90 minutes and DAPI staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (orb10106) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Gamma-Adaptin (orb10106) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kD, Observed band size: 91 kD.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Rat brian, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: rat lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Gamma-Adaptin Polyclonal Antibody, Unconjugated (orb10106) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

AP1G1

UniProt

O43747