GAK Antibody

• Catalog Number: orb1523309
• Category: Antibodies
• Description: GAK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: Between 675 and 725
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GAK
• UniProt ID: O14976
• NCBI: NP_005246.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: auxilin-2; cyclin-G-associated kinase; DNAJ26; DNA
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse GAK by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody orb1523309 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human GAK by western blot. Samples: Whole cell lysate (10 µg) from GaMG, HeLa, HEK293T, Jurkat, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAK antibody orb1523309 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.