GABRA6 Antibody

SKU: orb1248389

Description

GABRA6 Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 75kDa and 37kDa in Human Cerebellum lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.0kDa according to NP_000802.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: ENALGKLEVEGN-C
• Target: GABRA6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABRA6, gamma-aminobutyric acid (GABA) A receptor, alpha 6, MGC116903, MGC116904, GABA subunit A receptor alpha 6, GABA(A) receptor subunit alpha-6, gamma-aminobutyric acid receptor subunit alpha-6

orb1248389 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GABRA6

UniProt

Q16445

RefSeq (Protein):NP_000802.2