GABA Transporter 3 Rabbit Polyclonal Antibody

SKU: orb156998

Description

GABA Transporter 3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GABA Transporter 3/GAT 3 (531-632/632aa)
• Target: SLC6A11
• Molecular Weight: 71 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABT3; GAT-3; GAT3; S6A11_HUMAN; Slc6a11; Sodium-and chloride-dependent GABA transporter 3; SOLUTE CARRIER FAMILY 6(NEUROTRANSMITTER TRANSPORTER, GABA), MEMBER 11; Solute carrier family 6 member 11.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (orb156998), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: A431 (human) cell Lysate at 40 ug, Primary: Anti-GABA Transporter 3 (orb156998) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 71 kD.

Quick Database Links

Gene Symbol

SLC6A11

UniProt

P48066