G6PC Rabbit Polyclonal Antibody

• Catalog Number: orb6097
• Category: Antibodies
• Description: G6PC Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase alpha (81-180/357aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: G6PC
• UniProt ID: P35575
• RRID: AB_10929668
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: glucose-6-phosphatase, catalytic subunit; GSD1; AW
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mizuno, Tooru M. et al. Negative regulation of hepatic fat mass and obesity associated (Fto) gene expression by insulin Life Sci, 170, 50-55 (2017)

PubMed: 27914922

Applications

WB

Reactivity

Mouse

Zhang, Mian et al. Prevention of Injury-Induced Osteoarthritis in Rodent Temporomandibular Joint by Targeting Chondrocyte CaSR J Bone Miner Res, 34, 726-738 (2019)

PubMed: 30496623

Applications

WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Glucose 6 phosphatase alpha) Polyclonal Antibody, Unconjugated (orb6097) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human U937cell lysates, Lane 3: Human HL-60 cell lysates, Lane 4: Human HepG2 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Primary: Anti-Glucose 6 phosphatase alpha (orb6097) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 35 kDa.

U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with orb6097 Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).