FXYD5 antibody

• Catalog Number: orb18356
• Category: Antibodies
• Description: Goat polyclonal antibody to FXYD5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:8000
• Conjugation: Unconjugated
• MW: 19.5
• Target: Dysadherin
• Entrez: 53827
• Protein Sequence: GKCRQLSRLCRNHCR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FXYD5 antibody, anti dysadherin antibody, ant
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 38 kDa band in Human Spleen lysate and an approx 26 kDa in Human Lung lysate after 1 μg/ml antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa according to NP_054883 and NP_659003. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Expiration Date: 12 months from date of receipt.

orb18356 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.