FUT2 Rabbit Polyclonal Antibody

• Catalog Number: orb156968
• Category: Antibodies
• Description: FUT2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from Human FUT2 (231-343/343aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: FUT2
• UniProt ID: Q10981
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Fucosyltransferase 2; Se; SE2; SEC2; Secretor fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Yu Zhang 1, Zhihong Zhou 2, Zeming Zhang 3, Yan Liu 1, Wenting Ji 2, Jinglin Wang 4, Kaiping Wang 5, Qiang Li Lentinan mitigates ulcerative colitis via the IL-22 pathway to repair the compromised mucosal barrier and enhance antimicrobial defense Int J Biol Macromol ., (2025)

PubMed: 40054799

Applications

WB

Reactivity

Mouse

Wang, Zhe et al. FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response Redox Biol, 60, 102618 (2023)

PubMed: 36724577

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (FUT2) Polyclonal Antibody, Unconjugated (orb156968) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: SW480 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-FUT2 (orb156968) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 54 kD.