FusionRed Antibody

SKU: orb3188439

Description

Goat polyclonal antibody to FusionRed. FusionRed is a monomeric red fluorescent protein (~26–27 kDa) engineered from coral-derived fluorescent proteins originating from the sea anemone Entacmaea quadricolor. It is designed as a low-cytotoxicity, bright reporter suitable for fusion tagging, live-cell imaging, and long-term expression studies. FusionRed is a basic (constitutively fluorescent) protein with improved maturation and reduced phototoxicity compared to earlier red fluorescent proteins. It has a maximum excitation at 580 nm and a maximum emission at 608 nm. FusionRed-tagged proteins can be detected in Western blot at approximately 26–27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibody staining can enhance detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in studies of protein localization, trafficking, and live-cell imaging, especially where reduced phototoxicity is important.

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:500-1:5,000
• Reactivity: Other

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified recombinant fluorescent protein produced in E. coli.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FusionRed1, FusionRed2, FusionRed3, Far-Red antibody.

Anti-FusionRed Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 40 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.