FUS Rabbit Polyclonal Antibody

• Catalog Number: orb577521
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FUS
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FUS
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: FUS
• UniProt ID: P35637
• Protein Sequence: Synthetic peptide located within the following region: MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGY
• NCBI: NP_001010850
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: TLS, ALS6, ETM4, FUS1, POMP75, altFUS, HNRNPP2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. 53 kDa protein has extensive modification of arginines and contains a SUMO2 site.

Sample Type: Lane 1: Human MCF-7 cell lysateK562, Lane 2: Human THP-1 cell lysate, Lane 3: Human Hela cell lysate, Antibody Dilution: 1.0 ug/ml.

Rabbit Anti-FUS Antibody, Paraffin Embedded Tissue: Human alveolar cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

Rabbit Anti-FUS Antibody, Paraffin Embedded Tissue: Human Breast, Antibody Concentration: 5 ug/ml.

Rabbit Anti-FUS Antibody, Paraffin Embedded Tissue: Human Intestine, Cellular Data: Epithelial cells of intestinal villas, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

WB Suggested Anti-FUS Antibody, Positive Control: Lane 1: 5 ug mouse brain cytoplasm, Lane 2: 5 ug mouse brain nucleus, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti rabbit-IR-dye, Secondry Antibody Dilution: 1:10000.

WB Suggested Anti-FUS Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate. FUS is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.