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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. When rhFSTL1 / FRP is coated at 2 μg/mL (100 μl/well), the concentration of rhBMP-4 that produces 50% of the optimal binding response is found to be approximately 15-70 ng/mL. |
| Target | FSTL1 |
| Tag | His Tag |
| MW | 32.7 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Glu 21 - Ile 308 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Human FSTL1 Protein [orb2994687]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.5 KDa. Observed: 48 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FSTL1 Protein [orb2994642]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 33 KDa. Observed: 39 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FSTL1 Protein [orb2994643]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.8 KDa. Observed: 30-38 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse FSTL1 Protein [orb2994352]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 59.6 KDa. Observed: 70-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse FSTL1 Protein [orb2994353]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.5 KDa. Observed: 45-58 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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The purity of rh FSTL1/FRP was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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FSTL1 Recombinant Protein (orb1228050)
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