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Catalog Number | orb345056 |
---|---|
Category | Antibodies |
Description | Fructosyl-Amino Acid Oxidase antibody (Biotin) |
Clonality | Polyclonal |
Species/Host | Sheep |
Isotype | IgG |
Conjugation | Biotin |
Reactivity | Bacteria |
Form/Appearance | Lyophilized |
Concentration | 1.0 mg/mL |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Purity | Anti-Fructosyl-Amino Acid Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase [E.coli]. Cross reactivity against Fructosyl Amino Acid Oxidase from other sources is unknown. |
Immunogen | Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli] |
UniProt ID | Q8RIU8 |
Tested applications | ELISA, IP, WB |
Dilution range | ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000 |
Application notes | Anti-Fructosyl-Amino Acid Oxidase Antibody has been assayed against 1.0 µg of Fructosyl Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product. |
Antibody Type | Primary Antibody |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Alternative names | sheep anti-Fructosyl-Amino Acid Oxidase biotin con Read more... |
Note | For research use only |
NCBI | BAB91123.1 |
Expiration Date | 12 months from date of receipt. |