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Fructosyl-Amino Acid Oxidase Antibody Peroxidase Conjugated

SKU: orb345044

Description

Fructosyl-Amino Acid Oxidase antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IP, WB
Dilution rangeELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
ReactivityBacteria
Application Notes
Anti-Fructosyl-amino acid oxidase has been assayed against 1.0 µg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Key Properties

Antibody TypePrimary Antibody
HostSheep
ClonalityPolyclonal
IsotypeIgG
ImmunogenFructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
PurityFructosyl-amino acid oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli]. Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration1 mg/mL
DisclaimerFor research use only

Alternative Names

sheep anti-Fructosyl-Amino Acid Oxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Fructosyl-Amino Acid Oxidase Antibody

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Fructosyl-Amino Acid Oxidase Antibody Peroxidase Conjugated (orb345044)

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100 μg
$ 730.00
DispatchUsually dispatched within 5-10 working days
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