Fructosyl-Amino Acid Oxidase Antibody

SKU: orb345033

Description

Fructosyl-Amino Acid Oxidase antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:100, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Bacteria
• Application Notes:
  Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
• Purity: Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli]. Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.1 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase

Quick Database Links

UniProt

Q8RIU8

RefSeq:BAB91123.1