PFKM Antibody (Biotin)

SKU: orb344375

Description

Fructose-6-Phosphate Kinase antibody (Biotin)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Rabbit
• Application Notes:
  Anti-Fructose-6-Phosphate Kinase antibody is suitable for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]
• Target: PFKM
• Purity: Anti-Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Fructose-6-Phosphate Kinase Antibody biotin Conjugation, biotin Conjugation goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Quick Database Links

Gene Symbol

PFKM

UniProt

P00511

RefSeq:125128