Fructose-6-Phosphate Kinase Antibody

• Catalog Number: orb344336
• Category: Antibodies
• Description: Fructose-6-Phosphate Kinase antibody
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rabbit
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/ml
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-FRUCTOSE-6-PHOSPHATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.
• Immunogen: Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle
• UniProt ID: P00511
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:1,000 - 1:,5000, IF: 1:200 - 1:2,000, WB: 1:500 - 1:2,000
• Application notes: FRUCTOSE-6-PHOSPHATE KINASE antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Fructose-6-Phosphate Kinase Antibody, 6
• Note: For research use only
• NCBI: 125128

Confocal microscopy images using Biorbyt's anti-F6PK MAGP-2 antibody shows specific staining. Cultures of A7r5 VSM cells (rat aorta) were labeled with anti-F6PK (PFK) at 1:200 and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell.

Western blot analysis demonstrates the labeling specificity of the antibodies used for immunofluorescence. Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given.