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Catalog Number | orb344336 |
---|---|
Category | Antibodies |
Description | Fructose-6-Phosphate Kinase antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Rabbit |
Isotype | IgG |
Immunogen | Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:1,000 - 1:,5000, IF: 1:200 - 1:2,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Lyophilized |
Purity | Anti-FRUCTOSE-6-PHOSPHATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver. |
Conjugation | Unconjugated |
UniProt ID | P00511 |
NCBI | 125128 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Read more... |
Note | For research use only |
Application notes | FRUCTOSE-6-PHOSPHATE KINASE antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of cultures of A7r5 VSM cells (rat aorta) using Fructose-6-Phosphate Kinase antibody
IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Equine, Gallus, Porcine, Rabbit |
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