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| Catalog Number | orb765248 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Frizzled-7 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94 |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-2000;Flow Cyt 1:50-200;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Conjugation | Unconjugated |
| MW | 64 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-FZD7 antibody, anti-Frizzled-7 antibody, anti Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.
Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
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