Frizzled 10/CD350 Rabbit Polyclonal Antibody

• Catalog Number: orb156951
• Category: Antibodies
• Description: Frizzled 10/CD350 Rabbit Polyclonal Antibody
• Target: FZD10
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Frizzled 10/CD350 (101-200/581aa)
• UniProt ID: Q9ULW2
• MW: 68 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD 350; CD 350 antigen; CD350; Frizzled(Drosophila
• Research Area: Developmental Biology, G Protein-Coupled Receptors
• Note: For research use only

Protein: embryo (mouse) lysates at 30 ug, colon carcinoma (human) lysates at 30 ug, Primary: Anti-Frizzled 10/CD350 (orb156951) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 68kD, Observed band size: 68kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Frizzled 10/CD350 Polyclonal Antibody, Unconjugated (orb156951) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Frizzled 10/CD350 Polyclonal Antibody, Unconjugated (orb156951) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Frizzled 10/CD350 Polyclonal Antibody, Unconjugated (orb156951) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.