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FOXP3 Rabbit Polyclonal Antibody (PE)

Catalog Number: orb487179

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb487179
CategoryAntibodies
DescriptionFOXP3 Rabbit Polyclonal Antibody (PE)
TargetFoxP3
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationPE
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Sheep
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human FoxP3 (331-431/431aa)
Tested applicationsFC, IF
Dilution rangeFlow-Cyt=0.2ug/Test, IF=1:100-500
Antibody TypePrimary Antibody
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Alternative namesForkhead box protein P3; forkhead box P3; Scurfin;
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NoteFor research use only
FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (orb487179), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (orb487179), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-FoxP3/PE Conjugated antibody (orb487179), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: Mouse thymus. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (orb487179), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: Rat spleen. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (orb487179), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FOXP3 Rabbit Polyclonal Antibody (PE)

Blank control: Siha. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (orb487179), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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