FoxP1 Rabbit Polyclonal Antibody

• Catalog Number: orb10669
• Category: Antibodies
• Description: FoxP1 Rabbit Polyclonal Antibody
• Target: FOXP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse FoxP1 (581-705/705aa)
• UniProt ID: P58462
• RRID: AB_10750846
• MW: 79 kDa
• Tested applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FLJ23741; Fork head related protein like B; Forkhe
• Research Area: Cancer Research, Cell Biology, Forkhead box (FOX)
• Note: For research use only

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (FoxP1) polyclonal Antibody, Unconjugated (orb10669) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Pmel-1 splenocytes were stained with RABBIT ANTI-FOXP1 POLYCLONAL ANTIBODY, CONJUGATED. Accuri C6 flow cytometer was used for data acquisition.